Part:BBa_K3009035:Design

sfGFP (Amber inside chrom.) with MS2 loops

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1107
  Illegal EcoRI site found at 4055
  Illegal XbaI site found at 3608
  Illegal XbaI site found at 6147
  Illegal XbaI site found at 6256
  Illegal PstI site found at 6159
  Illegal PstI site found at 6229
  Illegal PstI site found at 6268
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1107
  Illegal EcoRI site found at 4055
  Illegal NheI site found at 1689
  Illegal NheI site found at 3572
  Illegal NheI site found at 3595
  Illegal NheI site found at 4049
  Illegal PstI site found at 6159
  Illegal PstI site found at 6229
  Illegal PstI site found at 6268
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1107
  Illegal EcoRI site found at 4055
  Illegal BamHI site found at 3988
  Illegal XhoI site found at 4509
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1107
  Illegal EcoRI site found at 4055
  Illegal XbaI site found at 3608
  Illegal XbaI site found at 6147
  Illegal XbaI site found at 6256
  Illegal PstI site found at 6159
  Illegal PstI site found at 6229
  Illegal PstI site found at 6268
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1107
  Illegal EcoRI site found at 4055
  Illegal XbaI site found at 3608
  Illegal XbaI site found at 6147
  Illegal XbaI site found at 6256
  Illegal PstI site found at 6159
  Illegal PstI site found at 6229
  Illegal PstI site found at 6268
  Illegal NgoMIV site found at 2622
  Illegal NgoMIV site found at 5917
  Illegal AgeI site found at 503
  Illegal AgeI site found at 1775
  Illegal AgeI site found at 2099
  Illegal AgeI site found at 3120
  Illegal AgeI site found at 3823
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4981
  Illegal BsaI site found at 5605
  Illegal SapI site found at 3805
  

Design Notes

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Source

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